Elisa kit evaluation for IGG and IGM antibodies to A-60 tubercular protein antigen

Elisa kit evaluation for IGG and IGM antibodies to A-60 tubercular protein antigen.

AIMS The purpose of this study is to evaluate the A-60 antigen-based enzyme-linked immuno sorbent assay (ELISA) test for its sensitivity, specificity, and other related statistical parameters. SETTINGS AND DESIGN Sera from 114 healthy volunteers, 105 bacteriologically confirmed cases of pulmonary tuberculosis (PTB), 59 sera from family contacts of PTB, and 40 sera from cases of lung infection...

Designing an ELISA Method for Measurement of Human IgG and IgM Antibodies against SARS-CoV-2

Background and purpose: Coronavirus disease 2019 (COVID-19) is a respiratory disease caused by severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2). In this study, an indirect ELISA method was designed to measure the human IgM and IgG antibodies against SARS-CoV-2. Materials and methods: Protein sequence of nucleocapsid antigen from SARS-CoV-2 was expressed in E. coli BL21 and then was...

HBc IgM ELISA Kit

Hepatitis B core Antigen (or HBcAg) is the major component of the core particles of Hepatitis B virus (or HBV). Particles have a size of 27 nm and contain a circular double-stranded DNA molecule, a specific DNA-polymerase and HBcAg. HBcAg is composed of a single polypeptide of about 17 kD that is released upon disaggregation of the core particles; the antigen contains at least one immunological...

Value of ELISA IgG and IgM Antibodies using A60 Antigen in the Diagnosis of Tuberculosis

Objectives: To evaluate a new Modified Anda-TB IgG ELISA test for the serodiagnosis of Mycobacterium tuberculosis in patients and controls. Settings and duration: The study was carried out between January to December 2010 at the Microbiology Department, Umm Al-Qura University, Makkah. Patients and Methods: The Anda IgG and IgM ELISA tests were run on 674 patient’s sera which included 378 active...

Dengue Virus IgM Elisa kit